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University of Balamand > Academics > Research > Seminars > Jihad Hawi

A New Model of Cardiovascular Disease

Wednesday, April 9, 2008 from 12:30 PM till 2:00 PM at the Jacobo Auditorium

SUBMITTED BY: Mr. Jihad Hawi
Faculty of Medicine
University of Balamand

ABSTRACT: Techniques in myocardial protection during open-heart surgery have been largely based on experimental models using healthy hearts. Healthy hearts are likely to have different characteristics to diseased hearts, and therefore these cardio-protective techniques may be inappropriate in a clinical setting. An established strain, the apolipoprotein E knockout (apoE‑/-) mouse has been used by Dr Jackson’s group in Bristol to provide the first mouse model of “atherosclerosis in the brachiocephalic artery with frank evidence of plaque rupture”. This model of apoE-/- mouse with high fat feeding is a model of atherosclerosis that displays similar characteristics to human disease and has been taken a step further to show that hearts of these male mice are ischaemic, show extensive coronary disease and suffer infarct. This is in complete contrast to their littermates, male apoE-/- mice fed normal diet. Male and female apoE-/- and wild-type(C57/129) mice were used in this study. Half of the mice were fed a standard chow diet and half were fed a western-type diet. After 24 weeks feeding, animals were terminated, hearts isolated and used for either isolation of myocytes or for Langendorff perfusion. A model of oxidative stress has been developed to compare vulnerability of isolated myocytes. Histological studies were performed to determine the extent of coronary disease in female apoE-/- and wild type hearts.
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